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Mouse抗原名称
CD15分子别名
Stage-Specific Embryonic Antigen 1; X-hapten; Lewis X; 3-FAL; SSEA1; SSEA-1细胞定位
Cell membrane克隆号
MC-480抗体类型
Mouse mAb抗体同种型
IgM,k反应种属 ?
Hu, Ms阳性样本
Human Peripheral Blood cells浓度
0.2 mg/ml标记
FITC性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 1.25μl per million cells in 100μl volume Hu, Ms
CD15, also known as Lewis X or SSEA-1, is a carbohydrate epitope (Galβ1→4[Fucα1→3]GlcNAc) carried primarily on glycoproteins and glycolipids of myeloid cells, granulocytes, and certain lymphocyte subsets; it serves as a key adhesion molecule that mediates selectin-dependent leukocyte rolling during inflammation, acts as a marker of mature neutrophils and Hodgkin/Reed-Sternberg cells, and its differential expression is exploited in immunohistochemistry to distinguish myeloid leukemias from lymphoid malignancies and to identify stem-cell populations in normal and neoplastic tissues.
流式分析
Flow cytometric analysis of human CD15 expression on human peripheral blood cells. Human peripheral blood cells were stained with either FITC Mouse IgM, k Isotype Control (left panel) or SDT FITC Mouse Anti-Human CD15 Antibody (right panel) at 1.25 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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