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宿主来源
Rat抗原名称
IL-7Rα (CD127)分子别名
Interleukin-7 receptor subunit alpha; IL-7 receptor subunit alpha; IL-7R subunit alpha; IL-7R-alpha; IL-7RA; Il7r细胞定位
MembraneAccession
P16872克隆号
A7R34抗体类型
Rat mAb抗体同种型
IgG2a,k反应种属 ?
Ms阳性样本
BALB/c mouse splenocytes纯化方式
Protein G浓度
0.2 mg/ml标记
PE-Cy7性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 1μg per million cells in 100μl volume Ms
Interleukin-7 receptor α chain (IL-7Rα, CD127) is a 75 kDa type-I transmembrane glycoprotein that forms the cytokine-binding subunit of both the IL-7 receptor (IL-7R) and the thymic stromal lymphopoietin receptor (TSLPR); upon binding IL-7 it heterodimerizes with the common gamma chain (γc, CD132) to initiate JAK1/JAK3-mediated phosphorylation of STAT5, thereby driving survival, proliferation and differentiation of T, B and innate lymphoid cells, while TSLP engagement pairs IL-7Rα with the TSLPR chain to modulate type-2 immunity.
流式分析
Flow cytometric analysis of mouse IL-7Rα (CD127) expression on BALB/c mouse splenocytes. BALB/c mouse splenocytes were stained with Brilliant Violet 421™ Rat Anti-Mouse CD3 Antibody and either PE-Cy7 Rat IgG2a, κ Isotype Control (left panel) or SDT PE-Cy7 Rat Anti-Mouse IL-7Rα (CD127) Antibody (right panel) at 5 μl/test treated with True-Stain Monocyte Blocker™. Total viable cells, as determined by Fixable Viability Dye 515 (S0D0013), were used for analysis. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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