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Mouse抗原名称
CD235a/b分子别名
Glycophorin-A; MN sialoglycoprotein; PAS-2; Sialoglycoprotein alpha; GYPA; GPA; MNS; Glycophorin-B; PAS-3; SS-active sialoglycoprotein; Sialoglycoprotein delta; GPB; GYPB细胞定位
Cell membraneAccession
P02724, P06028克隆号
HIR2抗体类型
Mouse mAb抗体同种型
IgG2b,k反应种属 ?
Hu阳性样本
Human Peripheral Blood cells纯化方式
Protein A浓度
0.2 mg/ml标记
APC性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 5μl per million cells in 100μl volume Hu
CD235a/b proteins, also known as glycophorins A and B, are type I transmembrane sialoglycoproteins expressed on human erythrocytes, erythroid precursor cells, and certain leukemic cell types. CD235a (glycophorin A) is a single-pass transmembrane glycoprotein with a molecular weight of approximately 10 kD, present in the cell membrane as a homodimer. It serves as the carrier for blood group M and N specificities and plays a role in reducing red blood cell aggregation in circulation. Additionally, CD235a acts as a receptor for certain viruses and bacterial adhesins, such as Sandei virus, parvovirus, and Streptococcus adhesin Hsa.
流式分析
Flow cytometric analysis of human CD235a/b expression on Human Peripheral Blood cells. Human Peripheral Blood cells were stained with Alexa Fluor® 488 Mouse Anti-Human CD45 Antibody and either Allophycocyanin Mouse IgG2b, κ Isotype Control (left panel) or Allophycocyanin Mouse Anti-Human CD235a/b Antibody (right panel) at 5 μl/test. Total viable cells, as determined by Fixable Viability Dye 452 (S0D0021), were used for analysis. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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