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宿主来源
Rat抗原名称
CD185分子别名
C-X-C chemokine receptor type 5; CXC-R5; CXCR-5; Burkitt lymphoma receptor 1 homolog; Blr1; Gpcr6; Cxcr5细胞定位
Cell membraneAccession
Q04683克隆号
S-R600抗体类型
Rat mAb抗体同种型
IgG2b,k反应种属 ?
Ms阳性样本
C57BL/6 mouse splenocytes纯化方式
Protein G浓度
0.2 mg/ml标记
PerCP-Cy5.5性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 1μg per million cells in 100μl volume Ms
CD185, also known as CXCR5 or Burkitt lymphoma receptor 1 (BLR1), is a G protein-coupled receptor with seven transmembrane regions. It belongs to the CXC chemokine receptor family and specifically binds to the chemokine CXCL13. This protein is crucial for the migration of B cells into B cell follicles in the spleen and Peyer's patches, and it is also essential for the function of follicular helper T cells. CD185 is expressed in mature B cells, follicular helper T cells, and certain types of lymphoma, including Burkitt's lymphoma. Additionally, it may play a role in maintaining cardiac structure under pressure overload and in the formation of ectopic mucosa-associated lymphoid tissue during chronic inflammation.
流式分析
Flow cytometric analysis of Mouse CD185 expression on C57BL/6 mouse splenocytes. C57BL/6 mouse splenocytes were stained with Brilliant Violet 421™ Rat Anti-Mouse CD19 Antibody and either PerCP-Cy5.5 Rat IgG2b, κ Isotype Control (left panel) or SDT PerCP-Cy5.5 Rat Anti-Mouse CD185 Antibody (right panel) at 5 μl/test treated with True-Stain Monocyte Blocker™. Total viable cells, as determined by Fixable Viability Dye 515 (S0D0013), were used for analysis. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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