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宿主来源
Mouse抗原名称
CD25分子别名
Peripheral myelin protein 22; PMP-22; Protein CD25; SR13 myelin protein; Schwann cell membrane glycoprotein (SAG); Cd25; Pmp22细胞定位
Cell membraneAccession
P25094克隆号
OX-39抗体类型
Mouse mAb抗体同种型
IgG1,k反应种属 ?
Rt阳性样本
SD Rat splenocytes treated 72h with 2.5μg/ml Cona纯化方式
Protein G浓度
0.2 mg/ml标记
PerCP-Cy5.5性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 1μg per million cells in 100μl volume Rt
CD25, also known as the interleukin-2 receptor alpha chain (IL-2Rα), is a 55 kDa type I transmembrane glycoprotein essential for immune regulation, forming part of the high-affinity IL-2 receptor complex (with IL-2Rβ and the common γ chain) that mediates IL-2 signaling in activated T cells, B cells, NK cells, and particularly in CD4+ regulatory T cells (Tregs), where its expression is critical for maintaining immune homeostasis and self-tolerance, and is dynamically upregulated upon T cell activation through transcriptional control involving NF-AT factors, while also existing in a soluble form (sCD25) that can modulate IL-2 availability and serve as a clinical biomarker in autoimmune diseases, infections, and cancers.
流式分析
Flow cytometric analysis of SD Rat splenocytes treated 72 h with 2.5 μg/ml ConA (right panel) or untreated (left panel) was stained with APC-Cy7 Mouse Anti-Rat CD4 Antibody and SDT PerCP-Cy5.5 Mouse Anti-Rat CD25 Antibody at 5 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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