Alexa Fluor® 647 Mouse Anti-Human CD16a Antibody (S-564-39)
Low affinity immunoglobulin gamma Fc region receptor III-A,IgG Fc receptor III-A,CD16-II,CD16a antigen,Fc-gamma RIII-alpha (Fc-gamma RIII,Fc-gamma RIIIa,FcRIII,FcRIIIa,FcgammaRIIIA),FcR-10,IgG Fc receptor III-2,CD16a,FCG3,FCGR3,IGFR3,FCGR3A
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宿主来源
Mouse抗原名称
CD16a分子别名
Low affinity immunoglobulin gamma Fc region receptor III-A; IgG Fc receptor III-A; CD16-II; CD16a antigen; Fc-gamma RIII-alpha (Fc-gamma RIII; Fc-gamma RIIIa; FcRIII; FcRIIIa; FcgammaRIIIA); FcR-10; IgG Fc receptor III-2; CD16a; FCG3; FCGR3; IGFR3; FCGR3A免疫原
Recombinant Protein细胞定位
Cell membrane, SecretedAccession
P08637克隆号
S-564-39抗体类型
Mouse mAb抗体同种型
IgG1,k反应种属 ?
Hu阳性样本
Human PBMC纯化方式
Protein G浓度
0.2 mg/ml标记
Alexa Fluor® 647性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 1.25μl per million cells in 100μl volume Hu
CD16, also known as FcγRIII, is a type of glycoprotein belonging to the immunoglobulin superfamily. It is divided into two subtypes: CD16a (FcγRIIIa) and CD16b (FcγRIIIb). CD16a is a transmembrane protein mainly expressed on natural killer (NK) cells, monocytes, and some T cell subsets, while CD16b is a GPI-anchored protein primarily found on neutrophils. CD16a can mediate antibody-dependent cell cytotoxicity (ADCC) by binding to the Fc region of IgG antibodies, which is crucial for the immune system to eliminate virus-infected cells and cancer cells. Additionally, CD16a is one of the core markers for defining NK cells and can promote their proliferation and secretion of cytotoxic cytokines. In cancer immunotherapy, CD16a is an important target protein, and some monoclonal antibody drugs are designed to enhance its binding ability to boost immune responses.
流式分析
Multiparameter flow cytometric analysis of human CD16a expression on human PBMC (human peripheral blood mononuclear cells). Human PBMC were stained with Brilliant Violet 421™ mouse anti-human CD3 Antibody and either Alexa Fluor® 647 Mouse IgG1, κ Isotype Control (left panel) or SDT Alexa Fluor® 647 Mouse Anti-Human CD16a Antibody (right panel) at 1.25 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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