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宿主来源
Rat抗原名称
CD21/CD35分子别名
Complement receptor type 2; Complement C3d receptor; Cr2; Cr1细胞定位
Cell membraneAccession
P19070克隆号
7E9抗体类型
Rat mAb抗体同种型
IgG2a,k反应种属 ?
Ms阳性样本
BALB/c mouse splenocytes纯化方式
Protein G浓度
0.5 mg/ml标记
Pacific Blue性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 0.25μg per million cells in 100μl volume Ms
CD21 (CR2) and CD35 (CR1) are key complement receptors predominantly expressed on B cells and follicular dendritic cells (FDCs). CD21, also known as the C3d receptor, binds to complement fragments like C3d and iC3b, playing a crucial role in B cell activation and regulation of immune responses. It also serves as the receptor for Epstein-Barr virus (EBV) on B cells. CD35, on the other hand, binds to C3b and C4b, facilitating phagocytosis and immune complex clearance. It helps in the activation of B cells and regulation of complement activity. Both receptors are involved in antigen presentation and immune complex internalization, contributing to both innate and adaptive immune responses.
流式分析
Flow cytometric analysis of Mouse CD21/CD35 expression on BALB/c mouse splenocytes. BALB/c mouse splenocytes were stained with Alexa Fluor® 647 Rat Anti-Mouse CD19 Antibody and either Pacific Blue™ Rat IgG2a, κ Isotype Control (left panel) or SDT Pacific Blue™ Rat Anti-Mouse CD21/CD35 antibody (right panel) at 0.5μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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