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宿主来源
Rat抗原名称
CD23分子别名
Low affinity immunoglobulin epsilon Fc receptor; Fc-epsilon-RII; Lymphocyte IgE receptor; Fcer2a; Fcer2细胞定位
Secreted, Cell membraneAccession
P20693克隆号
S-R672抗体类型
Rat mAb抗体同种型
IgG2a,k反应种属 ?
Ms阳性样本
BALB/c mouse splenocytes纯化方式
Protein G浓度
0.5 mg/ml标记
Pacific Blue性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 1μg per million cells in 100μl volume Ms
CD23, also known as the low-affinity IgE receptor (FcεRII), is a 45 kDa type II integral membrane glycoprotein that plays a crucial role in regulating IgE production and B-cell differentiation. It is expressed on various immune cells, including mature B cells, monocytes, eosinophils, follicular dendritic cells, and platelets. CD23 exists in two isoforms, CD23a and CD23b, which differ in their amino acid sequences and functions. The molecule can also be cleaved into a soluble form (sCD23) that retains IgE-binding capabilities.
流式分析
Flow cytometric analysis of Mouse CD23 expression on BALB/c mouse splenocytes. BALB/c mouse splenocytes were stained with Alexa Fluor® 647 Rat Anti-Mouse CD19 Antibody and either Pacific Blue™ Rat IgG2a, κ Isotype Control (left panel) or SDT Pacific Blue™ Rat Anti-Mouse CD23 Antibody (right panel) at 2 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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