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宿主来源
Mouse抗原名称
CD31分子别名
Platelet endothelial cell adhesion molecule; PECAM-1; Pecam; Pecam1细胞定位
Cell membraneAccession
Q3SWT0克隆号
TLD-3A12抗体类型
Mouse mAb抗体同种型
IgG1,k反应种属 ?
Rt阳性样本
SD Rat splenocytes纯化方式
Protein G浓度
0.5 mg/ml标记
Alexa Fluor® 488性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 1μg per million cells in 100μl volume Rt
CD31 (PECAM-1) is a 130 kDa type I transmembrane glycoprotein of the immunoglobulin superfamily, expressed constitutively on endothelial cells, platelets, monocytes, neutrophils and some T cells, where it localizes to intercellular junctions and functions as a mechanosensitive adhesion and signaling hub that orchestrates leukocyte transendothelial migration through homophilic CD31-CD31 interactions and heterophilic binding to partners such as CD177 and integrins, while simultaneously regulating endothelial permeability, angiogenic sprouting and vascular integrity via Src- and PI3K-mediated phosphorylation of its short cytoplasmic tail, making it a key biomarker for microvessel density in tumors and a checkpoint regulator of monocyte FcγR responses.
流式分析
Flow cytometric analysis of Rat CD31 expression on SD Rat splenocytes. SD Rat splenocytes were stained with Allophycocyanin Mouse Anti-Rat CD45RA Antibody and either Alexa Fluor® 488 Mouse IgG1, k Isotype Control (left panel) or SDT Alexa Fluor® 488 Mouse Anti-Rat CD31 Antibody (right panel) at 2 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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