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宿主来源
Rat抗原名称
CD226分子别名
CD226 antigen; Platelet and T-cell activation antigen 1; Pta1; Cd226细胞定位
Cell membraneAccession
Q8K4F0克隆号
S-R625抗体类型
Rat mAb抗体同种型
IgG2b,k反应种属 ?
Ms阳性样本
BALB/c mouse splenocytes纯化方式
Protein G浓度
0.5 mg/ml标记
Biotin性状
Liquid缓冲体系
PBS pH7.4, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 1μg per million cells in 100μl volume Ms
CD226, also known as DNAM-1, is a transmembrane glycoprotein in the immunoglobulin superfamily that functions as a key co-stimulatory receptor on T cells, NK cells, monocytes, and platelets, where it binds the nectin-family ligands CD155 and CD112 to trigger phosphorylation of its intracellular tyrosine and serine residues, thereby recruiting LFA-1 and activating Src, PI3K-AKT, VAV1-ERK, and PLCγ2 signaling cascades that enhance immune synapse formation, cytotoxic granule release, and production of IFN-γ and other cytokines, thus promoting antiviral and antitumor immunity; its expression correlates with improved outcomes in cancer immunotherapy, while its genetic polymorphisms or down-regulation are linked to autoimmune diseases and tumor immune evasion, making CD226 an emerging target for immunomodulatory therapies.
流式分析
Flow cytometric analysis of Mouse CD226 expression on BALB/c mouse splenocytes. BALB/c mouse splenocytes were stained with Alexa Fluor® 488 Rat Anti-Mouse CD8α Antibody (S0B8041) and either Biotin Rat IgG2b, k Isotype Control (left panel) or SDT Biotin Rat Anti- Mouse CD226 Antibody (right panel) at 2 μl/test followed by Sav-iFluor 488. Total viable cells, as determined by Fixable Viability Dye 452 (S0D0021), were used for analysis. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
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