分享产品介绍 评论(0)
宿主来源
Mouse抗原名称
CD83分子别名
CD83 antigen; hCD83; B-cell activation protein; Cell surface protein HB15细胞定位
MembraneAccession
Q01151克隆号
HB15e抗体类型
Mouse mAb抗体同种型
IgG1,k反应种属 ?
RhMk, CyMk, Hu阳性样本
Human peripheral blood monocyte-derived dendritic cells纯化方式
Protein G浓度
0.2 mg/ml标记
FITC性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 5μl per million cells in 100μl volume Hu
CD83 is a ~45 kDa, heavily glycosylated type-I transmembrane glycoprotein of the immunoglobulin superfamily that is encoded on human chromosome 6 and expressed chiefly on mature dendritic cells, Langerhans cells and activated lymphocytes, where it serves as a key maturation marker whose extracellular V-set Ig-like domain can dimerize via a membrane-proximal cysteine and whose membrane-bound form drives potent T-cell stimulation while its proteolytically released soluble counterpart (sCD83) exerts potent immunosuppressive effects by inhibiting dendritic-cell maturation and cytokine production, thereby modulating both the initiation and resolution of adaptive immune responses.
流式分析
Flow cytometric analysis of Human CD83 expression on human peripheral blood monocyte-derived dendritic cells. Human Peripheral blood monocyte-derived dendritic cells were stained with Phycoerythrin Mouse Anti-Human CD11c Antibody (S0B8092) and either Alexa Fluor® 488 Mouse IgG1, k Isotype Control (left panel) or SDT Alexa Fluor® 488 Mouse Anti-Human CD83 antibody (right panel) at 5 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
评论(0)