分享产品介绍 评论(0)
宿主来源
Mouse抗原名称
Perforin分子别名
Perforin-1; P1; Cytolysin; Lymphocyte pore-forming protein (PFP); PFP; PRF1细胞定位
Secreted, Cell membraneAccession
P14222克隆号
S-3064抗体类型
Mouse mAb抗体同种型
IgG2b,k反应种属 ?
Hu阳性样本
Human PBMC纯化方式
Protein A浓度
0.2 mg/ml标记
Biotin性状
Liquid缓冲体系
PBS pH7.4, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
ICFCM
稀释度
应用 稀释度 推荐种属 FCM 5μl per million cells in 100μl volume Hu
Perforin is a crucial protein involved in the immune response, primarily produced by cytotoxic T lymphocytes and natural killer cells. It functions as a pore-forming protein, inserting itself into the membranes of target cells, such as virus-infected or cancerous cells. Once integrated, perforin creates pores that disrupt the cell membrane's integrity, leading to osmotic lysis and ultimately killing the target cell. This mechanism is essential for the immune system's ability to eliminate harmful cells and maintain homeostasis.
流式分析
Flow cytometric analysis of Perforin expression in PBMC (Human peripheral blood mononuclear cells). Human PBMC were fixed and permeabilized with Foxp3 / Transcription Factor Staining Buffer Set. The cells were then stained with Alexa Fluor® 647 Mouse Anti-Human CD4 Antibody and either Biotin Mouse IgG2b, κ Isotype Control or SDT Biotin Mouse Anti-Human Perforin Antibody at 5μl/test followed by Sav-iFluor 488. Total viable cells, as determined by Fixable Viability Dye 452 (S0D0021), were used for analysis. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
评论(0)