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宿主来源
Mouse抗原名称
CD304分子别名
Neuropilin-1; Vascular endothelial cell growth factor 165 receptor; NRP; VEGF165R; NRP1细胞定位
Cell membrane, Cytoplasm, MitochondrionAccession
O14786克隆号
S-R695抗体类型
Mouse mAb抗体同种型
IgG2a,k反应种属 ?
Hu阳性样本
Human PBMC纯化方式
Protein A浓度
0.2 mg/ml标记
Biotin性状
Liquid缓冲体系
PBS pH7.4, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 5μl per million cells in 100μl volume Hu
CD304, also known as neuropilin-1 (NRP-1) or BDCA-4, is a type I transmembrane protein that plays crucial roles in various biological processes. It is a non-tyrosine kinase C-type lectin receptor, first identified on the cell membrane of plasmacytoid dendritic cells (pDCs). CD304 is involved in neuronal guidance during embryogenesis, angiogenesis, and immune responses. It serves as a co-receptor for vascular endothelial growth factor (VEGF) and members of the semaphorin family, interacting with plexins and VEGF receptors on neurons and endothelial cells . In the immune system, CD304 is expressed on natural Treg cells and may contribute to immunosuppression and tumor growth regulation . Additionally, CD304 is a valuable marker for distinguishing residual disease in B-cell acute lymphoblastic leukemia (B-ALL) and is widely used in minimal residual disease (MRD) detection.
流式分析
Flow cytometric analysis of human PBMC (human peripheral blood mononuclear cells) were stained with FITC Mouse Anti-Human CD123 Antibody and either Biotin Mouse IgG2a,k Isotype Control (left panel) or Biotin Mouse anti-Human CD304 antibody at 5μl/test (right panel) followed by Sav-PE. Total viable cells, as determined by Fixable Viability Dye 452 (S0D0021), were used for analysis. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
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