分享产品介绍 评论(0)
宿主来源
Mouse抗原名称
CD135分子别名
Receptor-type tyrosine-protein kinase FLT3; FL cytokine receptor; Fetal liver kinase-2 (FLK-2); Fms-like tyrosine kinase 3 (FLT-3); Stem cell tyrosine kinase 1 (STK-1); FLK2; STK1; FLT3细胞定位
MembraneAccession
P36888克隆号
S-3176抗体类型
Mouse mAb抗体同种型
IgG1,k反应种属 ?
Hu阳性样本
THP-1纯化方式
Protein G浓度
0.1 mg/ml标记
Biotin性状
Liquid缓冲体系
PBS pH7.4, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 1μl per million cells in 100μl volume Hu
CD135, also known as FLT3 (Fms-like tyrosine kinase 3), is a class III receptor tyrosine kinase that plays a critical role in the regulation of hematopoiesis by promoting the survival, proliferation, and differentiation of hematopoietic stem and progenitor cells upon binding to its ligand FLT3L; upon activation, CD135 undergoes dimerization and trans-phosphorylation, triggering downstream signaling cascades such as RAS/MAPK and PI3K/AKT pathways, which are essential for lymphoid lineage development and dendritic cell differentiation, while mutations—especially internal tandem duplications (FLT3-ITD)—lead to constitutive kinase activity, contributing to oncogenesis, particularly in acute myeloid leukemia (AML), where it is associated with poor prognosis and targeted by specific kinase inhibitors.
流式分析
Flow cytometric analysis of U-937 (Human histiocytic lymphoma monocyte, left) / THP-1 (Human monocytic leukemia monocyte, right) were stained with Biotin Mouse IgG1, κ (black) isotype control compared with SDT Biotin Mouse Anti-Human CD135 antibody at 1μl/test (red) followed by Sav-PE and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Negative control: U-937
评论(0)