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Alexa Fluor® 647 Mouse Anti-Human CD135 Antibody (S-3176)

Receptor-type tyrosine-protein kinase FLT3,FL cytokine receptor,Fetal liver kinase-2 (FLK-2),Fms-like tyrosine kinase 3 (FLT-3),Stem cell tyrosine kinase 1 (STK-1),FLK2,STK1,FLT3

价格 ￥1,000.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B8688
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产品介绍

产品介绍 评论(0)

产品规格

  • 宿主来源

    Mouse

  • 抗原名称

    CD135

  • 分子别名

    Receptor-type tyrosine-protein kinase FLT3; FL cytokine receptor; Fetal liver kinase-2 (FLK-2); Fms-like tyrosine kinase 3 (FLT-3); Stem cell tyrosine kinase 1 (STK-1); FLK2; STK1; FLT3

  • 细胞定位

    Membrane

  • Accession

    P36888

  • 克隆号

    S-3176

  • 抗体类型

    Mouse mAb

  • 抗体同种型

    IgG1,k

  • 反应种属 ?

    Hu

  • 阳性样本

    THP-1

  • 纯化方式

    Protein G

  • 浓度

    0.2 mg/ml

  • 标记

    Alexa Fluor® 647

  • 性状

    Liquid

  • 缓冲体系

    PBS, 1% BSA, 0.3% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, 2 to 8 °C as supplied

  • 应用

    FCM

  • 稀释度

    应用 稀释度 推荐种属
    FCM 5μl per million cells in 100μl volume Hu
背景介绍

  • CD135, also known as FLT3 (Fms-like tyrosine kinase 3), is a class III receptor tyrosine kinase that plays a critical role in the regulation of hematopoiesis by promoting the survival, proliferation, and differentiation of hematopoietic stem and progenitor cells upon binding to its ligand FLT3L; upon activation, CD135 undergoes dimerization and trans-phosphorylation, triggering downstream signaling cascades such as RAS/MAPK and PI3K/AKT pathways, which are essential for lymphoid lineage development and dendritic cell differentiation, while mutations—especially internal tandem duplications (FLT3-ITD)—lead to constitutive kinase activity, contributing to oncogenesis, particularly in acute myeloid leukemia (AML), where it is associated with poor prognosis and targeted by specific kinase inhibitors.

  • 流式分析

    • Flow cytometric analysis of U-937 (Human histiocytic lymphoma monocyte, left) / THP-1 (Human monocytic leukemia monocyte, right) were stained with Alexa Fluor® 647 Mouse IgG1, κ Isotype Control (black line histogram) or SDT Alexa Fluor® 647 Mouse anti-Human CD135(red line histogram) at 5μl/test, cells without incubation with primary antibody and secondary antibody (blue line histogram) was used as unlabeled control. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
      Negative control: U-937

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