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宿主来源
Mouse抗原名称
Aiolos分子别名
Zinc finger protein Aiolos; Ikaros family zinc finger protein 3; ZNFN1A3; IKZF3细胞定位
Cytoplasm, NucleusAccession
Q9UKT9克隆号
S-3264抗体类型
Mouse mAb抗体同种型
IgG1,k反应种属 ?
Hu阳性样本
Human PBMC纯化方式
Protein G浓度
0.2 mg/ml标记
Alexa Fluor® 488性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
ICFCM
稀释度
应用 稀释度 推荐种属 FCM 5μl per million cells in 100μl volume Hu
Aiolos (IKZF3) is a lymphoid-restricted zinc-finger transcription factor that partners with Ikaros to control B-cell differentiation, proliferation and apoptosis by remodeling chromatin and directly regulating genes such as BCL2 and BCL6; its expression peaks in peripheral blood leukocytes, spleen and thymus, is induced by TGFB1 and dioxin, and its loss-of-function or deletion is linked to B-cell lymphoma, chronic lymphocytic leukemia and multiple myeloma, while over-expression promotes migration and invasion of lung cancer cells and is being exploited as a target for PROTAC degraders.
流式分析
Flow cytometric analysis of Foxp3 / Transcription Factor Staining Buffer Set fixed and permeabilized Human PBMC (human peripheral blood mononuclear cells) were stained with Alexa Fluor® 488 Mouse IgG1, k Isotype Control (black line histogram) or SDT Alexa Fluor® 488 Mouse Anti Human Aiolos antibody (red line histogram) at 5μl/test. Total viable cells, as determined by Fixable Viability Dye 452 (S0D0021), were used for analysis. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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