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Mouse抗原名称
H-2Kd分子别名
H-2 class I histocompatibility antigen, K-D alpha chain; H-2K(D); H2-K; H2-K1细胞定位
MembraneAccession
P01902克隆号
SF1-1.1抗体类型
Mouse mAb抗体同种型
IgG2a,k反应种属 ?
Ms阳性样本
Balb/c mouse splenocytes纯化方式
Protein A浓度
0.2 mg/ml标记
Pacific Blue性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 5μl per million cells in 100μl volume Ms
H-2Kd is a murine MHC class I molecule expressed by BALB/c mice that presents endogenous 9-mer peptides—anchored by Tyr at position 2 and a hydrophobic residue at position 9—to CD8⁺ cytotoxic T cells, thereby initiating adaptive immune responses; its 2.6-Å crystal structure reveals a peptide-binding groove with five deep pockets (A–F) that impose an allele-specific motif, and it pairs non-covalently with β2-microglobulin for stability.
流式分析
Flow cytometric analysis of Mouse H-2Kd expression on BALB/c mouse splenocytes. BALB/c mouse splenocytes were stained with Pacific Blue™ Mouse IgG2a, κ Isotype Control (Black line histogram) or SDT Pacific Blue™ Mouse Anti-Mouse H-2Kd Antibody (Red line histogram) at 5μl/test, cells without incubation with primary antibody and secondary antibody (Blue line histogram) was used as unlabelled control. Total viable cells, as determined by Fixable Viability Dye 662 (S0D0016), were used for analysis. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
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