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宿主来源
Mouse抗原名称
CD1d分子别名
Antigen-presenting glycoprotein CD1d; R3G1; CD1D细胞定位
Cell membrane, Endoplasmic reticulum membraneAccession
P15813克隆号
51.1抗体类型
Mouse mAb抗体同种型
IgG2b,k反应种属 ?
Hu阳性样本
Human PBMC纯化方式
Protein A浓度
0.2 mg/ml标记
APC-Cy7性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 1.25μl per million cells in 100μl volume Hu
CD1d is a transmembrane glycoprotein that belongs to the group 2 CD1 family of non-classical MHC class I-like antigen-presenting molecules, expressed on dendritic cells, thymocytes, hepatocytes and many other tissues; it forms a heterodimer with β2-microglobulin, folds into a deep, hydrophobic groove made up of A′ and F′ pockets that cradle lipid tails while exposing the glycosyl headgroups of self or microbial glycolipids, and delivers these antigens to the invariant T-cell receptor of CD1d-restricted natural killer T (iNKT) cells, thereby rapidly triggering large bursts of cytokines such as IFN-γ and IL-4 that shape innate and adaptive immune responses against infections, cancer, and autoimmune diseases .
流式分析
Flow cytometric analysis of human CD1d expression on human PBMC (human peripheral blood mononuclear cells). Human PBMC were stained with Brilliant Violet 421™ Mouse Anti-Human CD19 antibody and either APC-Cy7 Mouse IgG2b, κ Isotype Control (left panel) or SDT APC-Cy7 Mouse Anti-Human CD1d Antibody (right panel) at 1.25 μl/test. Total viable cells, as determined by Fixable Viability Dye 515 (S0D0013), were used for analysis. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Flow cytometric analysis of human CD1d expression on human PBMC (human peripheral blood mononuclear cells). Human PBMC were stained with Brilliant Violet 421™ Mouse Anti-Human CD19 antibody and either APC-Cy7 Mouse IgG2b, κ Isotype Control (left panel) or SDT APC-Cy7 Mouse Anti-Human CD1d Antibody (right panel) at 1.25 μl/test. Total viable cells, as determined by Fixable Viability Dye 515 (S0D0013), were used for analysis. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
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