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宿主来源
Mouse抗原名称
CD45分子别名
Receptor-type tyrosine-protein phosphatase C; Leukocyte common antigen (L-CA); T200; CD45; PTPRC细胞定位
Cell membrane, SynapseAccession
P08575克隆号
HI100抗体类型
Mouse mAb抗体同种型
IgG2b,k反应种属 ?
Hu阳性样本
Human PBMC纯化方式
Protein A浓度
0.2 mg/ml标记
Alexa Fluor® 700性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 5μl per million cells in 100μl volume Hu
CD45RA is a splice variant of the CD45 protein, encoded by the PTPRC gene, and is a transmembrane glycoprotein with receptor-type protein tyrosine phosphatase activity. It contains the exon 4 in its structure, which distinguishes it from other CD45 isoforms like CD45RO. CD45RA is predominantly expressed on naïve T cells, indicating their potential for activation and differentiation into effector and memory T cells. Its unique extracellular domain, composed of fibronectin type III repeats, is crucial for modulating intracellular signaling pathways, particularly the T-cell receptor (TCR) signaling cascade. The presence of CD45RA on proliferating cells also serves as a marker for tracking clonal expansions in immune responses.
流式分析
Flow cytometric analysis of human CD45RA expression on human PBMC (human peripheral blood mononuclear cells). Human PBMC were stained with FITC Mouse Anti-Human CD45RO antibody and either Alexa Fluor® 700 Mouse IgG2b, κ Isotype Control (left panel) or SDT Alexa Fluor® 700 Mouse Anti-Human CD45RA Antibody (right panel) at 5 μl/test. Total viable cells, as determined by Fixable Viability Dye 452 (S0D0021), were used for analysis. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Flow cytometric analysis of human CD45RA expression on human PBMC (human peripheral blood mononuclear cells). Human PBMC were stained with FITC Mouse Anti-Human CD45RO antibody and either Alexa Fluor® 700 Mouse IgG2b, κ Isotype Control (left panel) or SDT Alexa Fluor® 700 Mouse Anti-Human CD45RA Antibody (right panel) at 5 μl/test. Total viable cells, as determined by Fixable Viability Dye 452 (S0D0021), were used for analysis. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
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