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宿主来源
Mouse抗原名称
H-2Kd分子别名
H-2 class I histocompatibility antigen, K-D alpha chain; H-2K(D); H2-K; H2-K1细胞定位
MembraneAccession
P01902克隆号
SF1-1.1抗体类型
Mouse mAb抗体同种型
IgG2a,k反应种属 ?
Ms阳性样本
BALB/c mouse splenocytes纯化方式
Protein A浓度
0.2 mg/ml标记
Alexa Fluor® 647性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 5μl per million cells in 100μl volume Ms
H-2Kd is a murine MHC class I molecule expressed by BALB/c mice that presents endogenous 9-mer peptides—anchored by Tyr at position 2 and a hydrophobic residue at position 9—to CD8⁺ cytotoxic T cells, thereby initiating adaptive immune responses; its 2.6-Å crystal structure reveals a peptide-binding groove with five deep pockets (A–F) that impose an allele-specific motif, and it pairs non-covalently with β2-microglobulin for stability.
流式分析
Flow cytometric analysis of Mouse H-2Kd expression on BALB/c mouse splenocytes. BALB/c mouse splenocytes were stained with Alexa Fluor® 647 Mouse IgG2a, κ Isotype Control (Black line histogram) or SDT Alexa Fluor® 647 Mouse Anti-Mouse H-2Kd Antibody (Red line histogram) at 5μl/test, cells without incubation with primary antibody and secondary antibody (Blue line histogram) was used as unlabelled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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