分享产品介绍 评论(0)
宿主来源
Mouse抗原名称
IL-21分子别名
Interleukin-21; Za11; IL21细胞定位
SecretedAccession
Q9HBE4克隆号
S-3523抗体类型
Rat mAb抗体同种型
IgG1,k反应种属 ?
Hu阳性样本
Th1 polarized human PBMC纯化方式
Protein G浓度
0.2 mg/ml标记
PE性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
ICFCM
稀释度
应用 稀释度 推荐种属 ICFCM 5μl per million cells in 100μl volume Hu
Interleukin-21 (IL-21) is a four-α-helical-bundle type I cytokine produced mainly by activated CD4⁺ T helper cells, NKT cells and TFH cells that signals through a heterodimeric receptor composed of the IL-21R and the common γ-chain (γc) to activate JAK1/JAK3–STAT1/STAT3 pathways, thereby orchestrating a broad immunomodulatory program that drives the differentiation, proliferation and effector function of B cells into long-lived plasma cells and memory B cells, promotes the development of Th17 and Tfh subsets while suppressing Treg stability, enhances cytotoxic activity and survival of CD8⁺ T cells and NK cells, and synergizes with IFN-γ or IL-15 to amplify antiviral and antitumor immunity, making it a key molecular hub whose dysregulation contributes to autoimmune pathologies such as systemic lupus erythematosus and type 1 diabetes and whose exogenous delivery or blockade is being therapeutically exploited in cancer immunotherapy, chronic viral infections and allergy.
流式分析
Flow cytometric analysis of IL-21 expression in human PBMC polarized with Th1 Polarization Kit, Human (UA090016). Th1 polarized human PBMC were harvested and restimulated for 5 hr with PMA and Ionomycin in the presence of Brefeldin A (Right Panel) or unstimulated (Left Panel).
The cells were harvested and fixed with 4% PFA and permeabilized with Intracellular Fixation & Permeabilization Buffer Set. The cells were then stained with Alexa Fluor® 647 Mouse Anti-Human CD4 and SDT PE Rat Anti-Human IL-21 Antibody at 5μl/test. Total viable cells, as determined by Fixable Viability Dye 452 (S0D0021), were used for analysis. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software."
评论(0)