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宿主来源
Rat抗原名称
Siglec-E分子别名
Sialic acid binding Ig-like lectin E; Siglece; Siglecl1细胞定位
MembraneAccession
Q6PJ50克隆号
S-3881抗体类型
Rat mAb抗体同种型
IgG2b反应种属 ?
Ms阳性样本
BALB/c mouse splenocytes纯化方式
Protein G浓度
0.2 mg/ml标记
Biotin性状
Liquid缓冲体系
PBS pH7.4, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 5μl per million cells in 100μl volume Ms
Siglec-E, the murine orthologue of human Siglec-9, is a sialic acid-binding immunoglobulin-like lectin expressed predominantly on myeloid cells (macrophages, neutrophils, dendritic cells) that functions as a critical immunosuppressive checkpoint by recruiting SHP-1/2 phosphatases upon engagement of sialoglycan ligands, thereby attenuating TLR4- and TRIF-dependent signaling, dampening NF-κB activation and pro-inflammatory cytokine production, and exerting neuroprotective and anti-atherogenic effects through inhibition of microglial activation and CD36-mediated oxidized LDL uptake.
流式分析
Flow cytometric analysis of Mouse Siglec-E expression on BALB/c mouse splenocytes. BALB/c mouse splenocytes were stained with Pacific Blue™ Rat Anti-Mouse Ly-6G/Ly-6C antibody and either Biotin Rat IgG2b Isotype Control (Left panel) or SDT Biotin Rat Anti-Mouse Siglec-E Antibody (Right panel) at 5 μl/test followed by Sav-PE. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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