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宿主来源
Mouse抗原名称
CD86分子别名
T-lymphocyte activation antigen CD86; Activation B7-2 antigen; B70; BU63; CTLA-4 counter-receptor B7.2; FUN-1; CD28LG2细胞定位
Cell membraneAccession
P42081克隆号
IT2.2抗体类型
Mouse mAb抗体同种型
IgG2b,k同型对照
S0B5779反应种属 ?
RhMk, CyMk, Hu阳性样本
Human PBMC纯化方式
Protein A浓度
0.2 mg/ml标记
APC性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 1.25μl per million cells in 100μl volume Hu, RhMk, CyMk
CD86, also known as B7-2, is a co-stimulatory molecule expressed on the surface of antigen-presenting cells (APCs) such as dendritic cells, macrophages, and B cells. It plays a crucial role in the immune response by interacting with CD28 on T cells to provide a necessary co-stimulatory signal for T cell activation, proliferation, and differentiation. Additionally, CD86 can also bind to CTLA-4, an inhibitory receptor on T cells, thereby regulating immune responses and maintaining immune homeostasis. This dual functionality makes CD86 a key target in immunotherapy research, particularly in the development of cancer treatments and autoimmune disease management strategies.
流式分析
Flow cytometric analysis of Human CD86 expression on human PBMC (human peripheral blood mononuclear cell). Human PBMC were stained with Brilliant Violet 421™ Mouse Anti-Human CD14 antibody and either APC Mouse IgG2b, κ Isotype Control (Left panel) or SDT APC Mouse Anti-Human CD86 Antibody (Right panel) at 1.25 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Flow cytometric analysis of CD86 expression on Rhesus monkey peripheral blood cells. Rhesus macaque peripheral blood cells were stained with APC Mouse IgG2b, κ Isotype Control (left panel) or SDT APC Mouse Anti- Human CD86 Antibody (right panel) at 1.25 μl/test. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Flow cytometric analysis of CD86 expression on Cynomolgus monkey peripheral blood cells. Cynomolgus macaque peripheral blood cells were stained with APC Mouse IgG2b, κ Isotype Control (left panel) or SDT APC Mouse Anti- Human CD86 Antibody (right panel) at 1.25 μl/test. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
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