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宿主来源
Mouse抗原名称
IL-9分子别名
Interleukin-9; Cytokine P40; T-cell growth factor P40; IL9细胞定位
SecretedAccession
P15248克隆号
S-2920抗体类型
Mouse mAb抗体同种型
IgG2b,k反应种属 ?
Hu阳性样本
Th2 polarized human PBMC纯化方式
Protein A浓度
0.2 mg/ml标记
Alexa Fluor® 488性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
ICFCM
稀释度
应用 稀释度 推荐种属 ICFCM 5μl per million cells in 100μl volume Hu
Interleukin-9 (IL-9) is a 14 kDa glycoprotein cytokine primarily produced by activated Th2, Th9, and innate lymphoid cells that signals through a heterodimeric receptor composed of the IL-9Rα chain and the common γ-chain, thereby activating JAK/STAT, PI3K/AKT, and MAPK pathways to exert pleiotropic effects on hematopoietic and epithelial cells, promoting mast-cell proliferation and differentiation, enhancing mucus production by goblet cells, modulating B-cell responses, and contributing to the pathogenesis of allergic asthma, atopic dermatitis, and certain hematologic malignancies, while also exerting context-dependent anti-tumor or pro-tumor activities depending on the cellular milieu.
流式分析
Flow cytometric analysis of IL-9 expression in human PBMC polarized with Th2 Polarization Kit, Human (UA090018). Th2 polarized human PBMC were harvested and restimulated for 5 hr with PMA and Ionomycin in the presence of Brefeldin A (Right Panel) or unstimulated (Left Panel).
The cells were harvested and fixed with 4% PFA and permeabilized with Intracellular Fixation & Permeabilization Buffer Set. The cells were then stained with PE Mouse Anti-Human CD4 and SDT Alexa Fluor® 488 Mouse Anti-Human IL-9 Antibody at 5μl/test. Total viable cells, as determined by Fixable Viability Dye 452 (S0D0021), were used for analysis. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software."
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