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MUC1/EMA Mouse mAb (PE Conjugate) (S-777-37)

Mucin-1,Breast carcinoma-associated antigen DF3,Cancer antigen 15-3 (CA 15-3),Carcinoma-associated mucin,Episialin,H23AG,CD227,PUM

价格 ￥1,110.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B8558
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产品介绍

产品介绍 评论(0)

产品规格

  • 宿主来源

    Mouse

  • 分子别名

    Mucin-1; Breast carcinoma-associated antigen DF3; Cancer antigen 15-3 (CA 15-3); Carcinoma-associated mucin; Episialin; H23AG; CD227; PUM

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Cell membrane, Cytoplasm

  • Accession

    P15941

  • 克隆号

    S-777-37

  • 抗体类型

    Mouse mAb

  • 抗体同种型

    IgG1,k

  • 反应种属 ?

    Hu, Rt

  • 纯化方式

    Protein G

  • 浓度

    1 mg/ml

  • 标记

    PE

  • 性状

    Liquid

  • 缓冲体系

    PBS, 1% BSA, 0.3% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, 2 to 8 °C as supplied

  • 应用

    ICFCM

    ICC ?

    IF ?

  • 稀释度

    应用 稀释度 推荐种属
    ICC 1:500 Hu
    IF 1:200 Hu, Rt
    ICFCM 1:1000 Hu
背景介绍

  • MUC1, also historically known as Epithelial Membrane Antigen (EMA), is a large transmembrane glycoprotein belonging to the mucin family that is normally expressed on the apical surface of most secretory epithelial cells, where its heavily O-glycosylated extracellular domain serves critical protective and lubricating functions while maintaining cell polarity; however, in malignant transformations across various carcinomas (including breast, lung, pancreatic, and ovarian cancers), MUC1 undergoes profound aberrations characterized by underglycosylation, loss of polarized distribution, and overexpression, which expose novel peptide epitopes and facilitate tumor progression through mechanisms such as shielding cancer cells from immune surveillance, promoting metastasis via interactions with selectins, and activating oncogenic signaling pathways like NF-κB and β-catenin, thereby making it not only a widely used diagnostic immunohistochemical marker for epithelial-derived tumors but also a prominent target for therapeutic strategies including monoclonal antibodies, antibody-drug conjugates, and cancer vaccines.

  • 流式分析

    • Flow cytometric analysis of HCT116 (Human colorectal carcinoma epithelial cell, left) / T-47D (Human ductal breast epithelial tumor epithelial cell, right) stained with PE Mouse IgG1, k Isotype Control (black line histogram) and SDT PE MUC1/EMA Mouse mAb (red line histogram) at 1/1000 dilution (0.1μg), cells without incubation with primary antibody and secondary antibody (blue line histogram) was used as unlabeled control. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
      Negative control: HCT116

  • 免疫细胞化学

    • ICC shows positive staining in T-47D cells (top panel) and negative staining in HCT116 cells (below panel). Anti-MUC1/EMA (PE Conjugate) antibody was used at 1/500 dilution (Red) and incubated overnight at 4°C. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).

  • 免疫荧光

    • IF shows positive staining in paraffin-embedded human colon. Anti-MUC1/EMA (PE Conjugate) antibody was used at 1/200 dilution (Red) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

    • IF shows positive staining in paraffin-embedded rat stomach. Anti-MUC1/EMA (PE Conjugate) antibody was used at 1/200 dilution (Red) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

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