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宿主来源
Rat抗原名称
IL-4分子别名
Interleukin-4; B-cell stimulatory factor 1 (BSF-1); Binetrakin; Lymphocyte stimulatory factor 1; Pitrakinra; IL4细胞定位
SecretedAccession
P05112克隆号
MP4-25D2抗体类型
Rat mAb抗体同种型
IgG1反应种属 ?
Hu阳性样本
Th2 polarized Human PBMC纯化方式
Protein G浓度
0.2 mg/ml标记
FITC性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
ICFCM
稀释度
应用 稀释度 推荐种属 ICFCM 5μl per million cells in 100μl volume Hu
Interleukin-4 (IL-4) is a 15–20 kDa, glycosylated, four-α-helical cytokine secreted primarily by Th2-polarized CD4⁺ T cells, mast cells, eosinophils and basophils that signals through a heterodimeric receptor complex composed of the IL-4Rα chain (shared with IL-13) and either the common γ-chain (type I receptor, hematopoietic cells) or IL-13Rα1 (type II receptor, non-hematopoietic cells) to activate STAT6-dependent transcriptional programs, thereby orchestrating the switch of B cells to IgE and IgG1 production, driving alternative activation of macrophages (M2), expanding Th2 and Treg subsets, suppressing Th1/Th17 responses, and exerting critical homeostatic and pathological roles in allergy, asthma, helminth immunity, tissue repair and tumor immune evasion.
流式分析
Flow cytometric analysis of IL-4 expression in Human PBMC polarized with Th2 Polarization Kit, Human (UA090018). Th2 polarized Human PBMC were harvested and restimulated for 5 hr with PMA and Ionomycin in the presence of Brefeldin A (Right Panel) or unstimulated (Left Panel).
The cells were harvested and fixed with 4% PFA and permeabilized with Intracellular Fixation & Permeabilization Buffer Set. The cells were then stained with PE Mouse Anti-Human CD4 and SDT FITC Rat Anti-Human IL-4 Antibody at 5μl/test. Total viable cells, as determined by Fixable Viability Dye 452 (S0D0021), were used for analysis. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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