Alexa Fluor® 488 Mouse Anti-Human CD340 Antibody (S-3018)
Receptor tyrosine-protein kinase erbB-2,Metastatic lymph node gene 19 protein (MLN 19),Proto-oncogene Neu,Proto-oncogene c-ErbB-2,Tyrosine kinase-type cell surface receptor HER2,p185erbB2,HER2,MLN19,NEU,NGL,ERBB2
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宿主来源
Mouse抗原名称
CD340分子别名
Receptor tyrosine-protein kinase erbB-2; Metastatic lymph node gene 19 protein (MLN 19); Proto-oncogene Neu; Proto-oncogene c-ErbB-2; Tyrosine kinase-type cell surface receptor HER2; p185erbB2; HER2; MLN19; NEU; NGL; ERBB2细胞定位
Cell membraneAccession
P04626克隆号
S-3018抗体类型
Mouse mAb抗体同种型
IgG1,k反应种属 ?
Hu阳性样本
SK-BR-3纯化方式
Protein G浓度
0.2 mg/ml标记
Alexa Fluor® 488性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 5μl per million cells in 100μl volume Hu
CD340, also termed HER2/ErbB2, is a 1,255-amino-acid, 185 kDa glycosylated type-I trans-membrane receptor tyrosine kinase that lacks its own ligand-binding domain yet serves as the dimerization partner of choice for the other epidermal growth-factor receptors; upon hetero-dimerization it triggers potent downstream signaling cascades (MAPK, PI3K–Akt, PLC-γ, STAT) that drive proliferation, survival and microtubule stabilization, is frequently amplified or over-expressed on the surface of breast, ovarian and other epithelial tumours, and is therefore a key therapeutic target for monoclonal antibodies such as trastuzumab and pertuzumab.
流式分析
Flow cytometric analysis of Human CD340 expression on SK-BR-3 cells. Cells from the SK-BR-3 (Human breast adenocarcinoma epithelial cell, Right) or MCF7 (Human breast adenocarcinoma epithelial cell, Left) was stained with either Alexa Fluor® 488 Mouse IgG1, κ Isotype Control (Black line histogram) or SDT Alexa Fluor® 488 Mouse Anti-Human CD340 antibody (Red line histogram) at 5 μl/test, cells without incubation with primary antibody and secondary antibody (Blue line histogram) was used as unlabelled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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