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宿主来源
Armenian hamster抗原名称
CD95分子别名
Tumor necrosis factor receptor superfamily member 6; Apo-1 antigen; Apoptosis-mediating surface antigen FAS; FASLG receptor; Apt1; Tnfrsf6; Fas细胞定位
Cell membraneAccession
P25446克隆号
S-R535抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Ms阳性样本
C57BL/6 mouse thymocytes纯化方式
Protein G浓度
0.2 mg/ml标记
Alexa Fluor® 647性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 1.25μl per million cells in 100μl volume Ms
CD95, also known as Fas or APO-1, is a 48 kDa type-I transmembrane receptor of the TNF-receptor superfamily that, upon trimerization by its cognate ligand CD95L (FasL), assembles the death-inducing signaling complex (DISC) by recruiting the adaptor FADD and procaspase-8/10, triggering a caspase cascade that culminates in apoptosis, thereby governing immune homeostasis, peripheral tolerance, and elimination of infected or oncogenic cells, while dysregulation of this pathway contributes to autoimmune lymphoproliferative syndrome, cancer immune evasion, and resistance to chemotherapy.
流式分析
Flow cytometric analysis of Mouse CD95 expression on C57BL/6 mouse thymocytes. C57BL/6 mouse thymocytes were stained with either Alexa Fluor® 647 Armenian hamster IgG Isotype Control (Left panel) or SDT Alexa Fluor® 647 Armenian hamster Anti-Mouse CD95 Antibody (Right panel) at 1.25 μl/test. Total viable cells, as determined by Fixable Viability Dye 452 (S0D0021), were used for analysis. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
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