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宿主来源
Rat抗原名称
CD370分子别名
C-type lectin domain family 9 member A; Dendritic cell natural killer lectin group receptor 1; Dngr-1; Clec9a细胞定位
MembraneAccession
Q8BRU4克隆号
7H11抗体类型
Rat mAb抗体同种型
IgG2b,k反应种属 ?
Ms阳性样本
C57BL/6 mouse splenocytes纯化方式
Protein A浓度
0.2 mg/ml标记
Biotin性状
Liquid缓冲体系
PBS pH7.4, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 5μl per million cells in 100μl volume Ms
CD370, also known as CLEC9A or DNGR-1, is a type II transmembrane glycoprotein expressed almost exclusively on human CD141⁺ (mouse CD8α⁺) dendritic cells and a minor subset of CD14⁺CD16⁻ monocytes; it functions as an endocytic pattern-recognition receptor that binds exposed filamentous actin on necrotic cell debris, triggering Syk–ITAM signaling that drives efficient cross-presentation of dead-cell antigens to cytotoxic T lymphocytes and thereby links necrosis sensing to initiation of adaptive immunity.
流式分析
Flow cytometric analysis of Mouse CD370 expression on C57BL/6 mouse splenocytes. C57BL/6 mouse splenocytes were stained with FITC Rat Anti-Mouse CD11c antibody and APC Rat Anti-Mouse CD8α either Biotin Rat IgG2b, κ Isotype Control (Left panel) or SDT Biotin Rat Anti- Mouse CD370 antibody (Right panel) at 5 μl/test followed by Sav-PE. Gated on CD8+ viable cells. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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