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宿主来源
Mouse抗原名称
CD40分子别名
Tumor necrosis factor receptor superfamily member 5; B-cell surface antigen CD40; Bp50; CD40L receptor; CDw40; TNFRSF5细胞定位
Secreted, Cell membraneAccession
P25942克隆号
HI40a抗体类型
Mouse mAb抗体同种型
IgG2b反应种属 ?
Hu阳性样本
Human PBMC纯化方式
Protein A浓度
0.2 mg/ml标记
Alexa Fluor® 488性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 5μl per million cells in 100μl volume Hu
CD40, also known as TNFRSF5 or Bp50, is a 48 kDa type I membrane glycoprotein belonging to the tumor necrosis factor receptor superfamily. It is widely expressed on various immune cells, including B cells, dendritic cells, and macrophages. The interaction between CD40 and its ligand, CD40L, is crucial for immune cell activation and regulation. Structurally, CD40 consists of a 62 amino acid cytoplasmic tail, a 22 amino acid transmembrane domain, and a 173 amino acid extracellular domain. This co-stimulatory protein plays a key role in B cell activation, proliferation, and differentiation, and is also involved in immune responses against infections and tumors. Due to its significant role in the immune system, CD40 has become an important therapeutic target, with various agonistic and antagonistic antibodies being developed to modulate its activity.
流式分析
Flow cytometric analysis of Human CD40 expression on Human PBMC (human peripheral blood mononuclear cells). Human PBMC cells were stained with Brilliant Violet 421™ Mouse Anti-Human CD19 antibody and either Alexa Fluor® 488 Mouse IgG2b Isotype Control (Left panel) or SDT Alexa Fluor® 488 Mouse Anti-Human CD40 Antibody (Right panel) at 5 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™software.
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