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宿主来源
Mouse抗原名称
IgE分子别名
Immunoglobulin heavy constant epsilon; Ig epsilon chain C region; Ig epsilon chain C region ND; IGHE细胞定位
Cell membrane, SecretedAccession
P01854克隆号
S-2853抗体类型
Mouse mAb抗体同种型
IgG1,k反应种属 ?
Hu阳性样本
Human PBMC纯化方式
Protein G浓度
0.2 mg/ml标记
Alexa Fluor® 488性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 5μl per million cells in 100μl volume Hu
IgE is an antibody isotype that, despite being the least abundant in serum, sits at the epicenter of type I hypersensitivity and anti-parasite immunity; generated by plasma cells in mucosal tissues, it binds with picomolar affinity to the tetrameric FcεRI on mast cells and basophils, priming them for rapid degranulation upon subsequent allergen encounter, thereby unleashing histamine, leukotrienes and cytokines that produce the clinical spectrum from urticaria to anaphylaxis, while also orchestrating eosinophil and macrophage responses against helminths; its half-life in plasma is brief (~2 days), yet it can remain cell-bound for weeks, and its levels are tightly regulated by IL-4, IL-13 and CD40L-mediated class-switching.
流式分析
Flow cytometric analysis of Human IgE expression on Human PBMC. Human PBMC (peripheral blood mononuclear cells) were stained with PE Mouse Anti-Human CD193 Antibody and either Alexa Fluor® 488 Mouse IgG1, κ Isotype Control (Left panel) or SDT Alexa Fluor® 488 Mouse Anti-Human IgE Antibody (Right panel) at 5 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ soft
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