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宿主来源
Mouse抗原名称
CD68分子别名
Macrosialin; Gp110细胞定位
Cell membraneAccession
P34810克隆号
Y1/82A抗体类型
Mouse mAb抗体同种型
IgG2b,k反应种属 ?
Hu阳性样本
Human PBMC纯化方式
Protein A浓度
0.2 mg/ml标记
PerCP-Cy5.5性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 ICFCM 1.25μl per million cells in 100μl volume Hu
CD68 is a heavily glycosylated, 110 kDa type I transmembrane glycoprotein of the lysosome-associated membrane protein (LAMP) family (also called LAMP-4) that is highly expressed on human monocytes, macrophages, osteoclasts, and other myeloid cells, where it predominantly localizes to lysosomes and endosomes and participates in phagocytosis, debris clearance, and inflammatory responses via its scavenger-receptor activity, while alternative splicing produces multiple isoforms and its presence on tumor cells and ability to bind selectins implicate it in metastasis, making it a widely used histological macrophage marker.
流式分析
Human PBMC (human peripheral blood mononuclear cells) were fixed with 4% PFA and permeabilization with the 0.1% Tween. Cells were then intracellularly stained with Brilliant Violet 421™ Mouse Anti-Human CD11b and either PerCP-Cy5.5 Mouse IgG2b, κ Isotype Control / (Left panel) or PerCP-Cy5.5 Mouse Anti-Human CD68 Antibody at 1.25μl/test / (Right panel) treated with True-Stain Monocyte Blocker™. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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