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宿主来源
Mouse抗原名称
CD47分子别名
Leukocyte surface antigen CD47; Antigenic surface determinant protein OA3; Integrin-associated protein (IAP); Protein MER6; MER6细胞定位
Cell membraneAccession
Q08722克隆号
B6H12抗体类型
Mouse mAb抗体同种型
IgG1,k同型对照
S0B0617反应种属 ?
RhMk, CyMk, Hu阳性样本
human peripheral blood leukocytes纯化方式
Protein G浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS pH7.4
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 1:200 Hu, RhMk, CyMk
CD47, also known as integrin-associated protein, is a ubiquitously expressed cell surface receptor that belongs to the immunoglobulin superfamily. It is widely known for its role in preventing macrophage-mediated phagocytosis by interacting with signal regulatory protein alpha (SIRPα) on phagocytic cells. This “don’t eat me” signal is crucial for the survival of healthy cells, but cancer cells often overexpress CD47 to evade immune attack. In addition to its role in immune regulation, CD47 is involved in various cellular processes, including cell adhesion, migration, and the regulation of autophagy and cell death. For instance, it can regulate the migration of immune cells like neutrophils and dendritic cells, and its interaction with thrombospondin-1 (TSP-1) can induce apoptosis in certain cell types. Due to its significant role in cancer immunotherapy, CD47 has become an important target for developing new therapeutic strategies.
流式分析
Flow cytometric analysis of human peripheral blood leukocytes labelling Human CD47 antibody at 1/200 dilution (1 μg) / (Right panel) compared with a mouse IgG1, κ Isotype control / (Left panel). Goat Anti-Mouse IgG Alexa Fluor® 488 was used as the secondary antibody.
Flow cytometric analysis of cynomolgus monkey peripheral blood cells labelled with CD47 antibody at 1/200 dilution (1 μg) / (right panel) compared with a mouse IgG1, κ Isotype control / (left panel). Goat Anti-Mouse IgG Alexa Fluor® 488 was used as the secondary antibody. Total viable cells, as determined by Fixable Viability Dye 452 (S0D0021), were used for analysis. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Flow cytometric analysis of rhesus monkey peripheral blood cells labelled with CD47 antibody at 1/200 dilution (1 μg) / (right panel) compared with a mouse IgG1, κ Isotype control / (left panel). Goat Anti-Mouse IgG Alexa Fluor® 488 was used as the secondary antibody. Total viable cells, as determined by Fixable Viability Dye 452 (S0D0021), were used for analysis. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
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