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宿主来源
Mouse抗原名称
CD3d分子别名
T-cell surface glycoprotein CD3 delta chain; T-cell receptor T3 delta chain; T3D; CD3D细胞定位
Cell membraneAccession
P04234克隆号
MEM-57抗体类型
Mouse mAb抗体同种型
IgG2a反应种属 ?
Hu阳性样本
Human peripheral blood leukocytes纯化方式
Protein A浓度
0.2 mg/ml标记
Alexa Fluor® 488性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 1.25μl per million cells in 100μl volume Hu
CD3d (CD3-delta) is a 19 kDa transmembrane glycoprotein that together with CD3ε, CD3γ and CD3ζ forms the CD3 signaling complex, which is tightly associated with the T-cell receptor (TCR) on thymocytes and mature T lymphocytes; upon antigen/MHC engagement the CD3 chains become phosphorylated on their cytoplasmic ITAMs by Lck and Fyn, initiating the cascade that leads to T-cell activation, proliferation and differentiation, while during thymic development CD3d is indispensable for correct assembly and surface expression of the pre-TCR/TCR complex and thus for positive selection of CD4⁺ and CD8⁺ T cells.
流式分析
Flow cytometric analysis of Human CD3d expression on human peripheral blood leukocytes. Human peripheral blood leukocytes were stained with Brilliant Violet 421™ Mouse Anti-Human CD19 antibody and either Alexa Fluor® 488 Mouse IgG2a Isotype Control (Left panel) or SDT Alexa Fluor® 488 Mouse Anti-Human CD3d Antibody (Right panel) at 1.25 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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