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Mouse抗原名称
Granzyme A分子别名
CTL tryptase; Cytotoxic T-lymphocyte proteinase 1; Fragmentin-1; Granzyme-1; Hanukkah factor (H factor; HF); CTLA3; HFSP; GZMA细胞定位
SecretedAccession
P12544克隆号
S-3175抗体类型
Mouse mAb抗体同种型
IgG1,k反应种属 ?
Hu阳性样本
Human PBMC纯化方式
Protein G浓度
0.2 mg/ml标记
Biotin性状
Liquid缓冲体系
PBS pH7.4, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
ICFCM
稀释度
应用 稀释度 推荐种属 ICFCM 5μl per million cells in 100μl volume Hu
Granzyme A (GZMA) is a 28 kDa serine protease stored in the cytotoxic granules of CD8⁺ T cells and NK cells; upon immunological synapse formation, it enters target cells together with perforin and cleaves after Lys or Arg residues to hydrolyze SET complex proteins (including the SET, APE1, and HMGB2 nucleases), thereby disrupting DNA repair and initiating a caspase-independent, oxidative DNA-damage cell death pathway that manifests as single-stranded DNA nicks, mitochondrial dysfunction, and plasma membrane perforation, while extracellularly it can process extracellular matrix components and cytokine precursors to modulate inflammation, vascular integrity, and antiviral immunity.
流式分析
Flow cytometric analysis of Human Granzyme A expression in Human PBMC. Human PBMC were fixed with 4% PFA and permeabilized with 0.1% Tween. The cells were then stained with Pacific Blue Mouse Anti-Human CD8 Antibody and either Biotin Mouse IgG1, κ Isotype Control (Left panel) or Biotin Mouse Anti-Human Granzyme A Antibody (Right panel) at 5 μl/test followed by Sav-iFluor 488. Total viable cells, as determined by Fixable Viability Dye 777 (S0D0026), were used for analysis. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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