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宿主来源
Mouse抗原名称
CD123分子别名
Interleukin-3 receptor subunit alpha; IL-3 receptor subunit alpha; IL-3R subunit alpha; IL-3R-alpha; IL-3RA; IL3R; IL3RA细胞定位
Cell membraneAccession
P26951克隆号
7G3抗体类型
Mouse mAb抗体同种型
IgG2a,k反应种属 ?
RhMk, CyMk, Hu阳性样本
Human PBMC纯化方式
Protein A浓度
0.2 mg/ml标记
Alexa Fluor® 700性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 1.25μl per million cells in 100μl volume Hu
CD123, also known as interleukin-3 receptor alpha chain (IL-3Rα), is a 75 kDa type-I transmembrane glycoprotein that pairs with the common β-subunit (CD131) to form the high-affinity IL-3 receptor, triggering JAK/STAT, PI3K/AKT and MAPK cascades that drive proliferation, survival and differentiation of hematopoietic stem/progenitor cells, plasmacytoid dendritic cells, basophils and eosinophils; it is constitutively over-expressed on blasts and leukemic stem cells in >90 % of acute myeloid leukemias, B-ALL and blastic plasmacytoid dendritic cell neoplasm, making it a key diagnostic biomarker and a validated target for monoclonal antibodies, antibody-drug conjugates, bispecific T-cell engagers and CAR-T/CAR-NK therapies.
流式分析
Flow cytometric analysis of Human CD123 expression on human PBMC (human peripheral blood mononuclear cell). Human PBMC were stained with Alexa Fluor® 488 Mouse Anti-Human HLA/DR antibody and either Alexa Fluor® 700 Mouse IgG2a, κ Isotype Control (Left panel) or SDT Alexa Fluor® 700 Mouse Anti-Human CD123 Antibody (Right panel) at 1.25 μl/test. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
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