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Rat抗原名称
CD252分子别名
Tumor necrosis factor ligand superfamily member 4; OX40 ligand (OX40L); Ox40l; Txgp1l; Tnfsf4细胞定位
MembraneAccession
P43488克隆号
S-R735抗体类型
Rat mAb抗体同种型
IgG2b,k反应种属 ?
Ms阳性样本
stimulated mouse splenocytes B cells纯化方式
Protein G浓度
0.2 mg/ml标记
Alexa Fluor® 647性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 1.25μl per million cells in 100μl volume Ms
CD252, also known as OX40 ligand (OX40L), is a membrane-bound cytokine of the tumor necrosis factor superfamily expressed on activated antigen-presenting cells that engages its receptor OX40 (CD134) on T cells to deliver a potent costimulatory signal driving T-cell proliferation, survival and effector cytokine production while also participating in B-cell/T-cell cross-talk and being exploited as a therapeutic target in cancer immunotherapy and autoimmune disease modulation.
流式分析
Flow cytometric analysis of mouse CD252 expression on stimulated mouse splenocytes B cells. Mouse splenocytes B cells were stimulated (3 days) in culture with Purified Anti-Mouse CD40 antibody and Goat Anti-Mouse IgM. The cells were then stained with either Alexa Fluor® 647 Rat IgG2b, κ Isotype Control or SDT Alexa Fluor® 647 Rat Anti-Mouse CD252 Antibody at 1.25 μl/test Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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