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宿主来源
Mouse抗原名称
CD152分子别名
Cytotoxic T-lymphocyte protein 4; Cytotoxic T-lymphocyte-associated antigen 4 (CTLA-4); CTLA4细胞定位
Cell membraneAccession
P16410克隆号
BNI3抗体类型
Mouse mAb抗体同种型
IgG2a,k反应种属 ?
Hu阳性样本
PHA treated human peripheral blood lymphocytes纯化方式
Protein G浓度
0.2 mg/ml标记
PerCP-Cy5.5性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
ICFCM
稀释度
应用 稀释度 推荐种属 ICFCM 1.25μl per million cells in 100μl volume Hu
CD152, also known as CTLA-4 (cytotoxic T-lymphocyte-associated protein 4), is a protein receptor that functions as an immune checkpoint. It is a member of the immunoglobulin superfamily and is expressed on the surface of T cells. The CD152 gene consists of 4 exons and encodes a protein with a signal peptide, an Ig-like domain, a transmembrane region, and a cytoplasmic domain. CD152 plays an essential role in regulating T cell activation by binding to its ligands CD80 and CD86, which are expressed on antigen-presenting cells. This binding can inhibit the over-activation of T cells, thus maintaining immune homeostasis.
流式分析
Flow cytometric analysis of CD152 expression in activated human PBMC (human peripheral blood mononuclear cell). Human PBMC stimulated 2 days with 10 μg/ml PHA (Right panel) or unstimulated (Left panel) was fixed with 4% PFA and permeabilization with the 0.1% Tween. The cells were then stained with Brilliant Violet 421™ Mouse Anti-Human CD3 and SDT PerCP-Cy5.5 Mouse Anti-Human CD152 Antibody at 1.25 μl/test treated with True-Stain Monocyte Blocker™. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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