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宿主来源
Rat抗原名称
IFNγ分子别名
Interferon gamma; IFN-gamma; Ifng细胞定位
SecretedAccession
P01580克隆号
XMG1.2抗体类型
Rat mAb抗体同种型
IgG1,k反应种属 ?
Ms阳性样本
Th1 polarized C57BL/6 mouse splenocytes纯化方式
Protein G浓度
0.2 mg/ml标记
FITC性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
ICFCM
稀释度
应用 稀释度 推荐种属 ICFCM 1.25μl per million cells in 100μl volume Ms
Interferon-gamma (IFNγ) is the signature cytokine of type II interferon, a 20–25 kDa glycoprotein homodimer that is synthesized and secreted primarily by activated T cells, NK cells and NKT cells; upon binding to its high-affinity receptor (IFNGR1/2) it triggers JAK1/JAK2-mediated phosphorylation of STAT1, launching a transcriptional program that up-regulates MHC class I and II, antigen-processing machinery, chemokines such as CXCL9/10/11, nitric-oxide and reactive-oxygen species, and hundreds of IFN-stimulated genes, thereby polarizing macrophages toward the microbicidal M1 phenotype, enhancing Th1 responses, orchestrating antiviral, antitumor and intracellular bacterial immunity, and exerting pro- or anti-inflammatory feedback depending on context; genetic or therapeutic blockade of IFNγ in mice and humans highlights its essential yet double-edged role in controlling infection, tumor surveillance, autoimmune diseases and immune-related adverse events.
流式分析
Flow cytometric analysis of IFNγ expression in C57BL/6 mouse splenocytes polarized with Th1 Polarization Kit, Mouse (UA090017). Th1 polarized C57BL/6 mouse splenocytes were harvested and restimulated for 5 hr with PMA and Ionomycin in the presence of Brefeldin A (Right Panel) or unstimulated (Left Panel).
The cells were harvested and fixed with 4% PFA and permeabilized with Intracellular Fixation & Permeabilization Buffer Set. The cells were then stained with SDT FITC Rat Anti-Mouse IFNγ Antibody at 1.25μl/test. Total viable cells, as determined by Fixable Viability Dye 452 (S0D0021), were used for analysis. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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