Biotin Rat Anti-Mouse TIM-3 (CD366) Antibody (RMT3-23)
Hepatitis A virus cellular receptor 2 homolog,HAVcr-2,T-cell immunoglobulin and mucin domain-containing protein 3 (TIMD-3),T-cell immunoglobulin mucin receptor 3 (TIM-3),T-cell membrane protein 3,Tim3,Timd3,Havcr2
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宿主来源
Rat抗原名称
TIM-3 (CD366)分子别名
Hepatitis A virus cellular receptor 2 homolog; HAVcr-2; T-cell immunoglobulin and mucin domain-containing protein 3 (TIMD-3); T-cell immunoglobulin mucin receptor 3 (TIM-3); T-cell membrane protein 3; Tim3; Timd3; Havcr2细胞定位
MembraneAccession
Q8VIM0克隆号
RMT3-23抗体类型
Rat mAb抗体同种型
IgG2a,k反应种属 ?
Ms阳性样本
CD3, CD28 antibody treated C57BL/6 mouse splenocytes纯化方式
Protein G浓度
0.2 mg/ml标记
Biotin性状
Liquid缓冲体系
PBS pH7.4, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 5μl per million cells in 100μl volume Ms
TIM-3 (CD366) is a trans-membrane immune checkpoint receptor in the Ig super-family whose extracellular domain binds phosphatidyl-serine, CEACAM-1 or galectin-9 ligands to trigger downstream signaling through its conserved cytoplasmic tyrosine motif, thereby attenuating T-cell, NK-cell and dendritic-cell activation while promoting regulatory T-cell function and myeloid tolerance, making its blockade a promising strategy for enhancing anti-tumor and anti-viral immunity across multiple cancers and chronic infections.
流式分析
Flow cytometric analysis of Mouse CD366 (TIM-3) expression on mouse CD3, CD28 antibody treated C57BL/6 mouse splenocytes. C57BL/6 mouse splenocytes were either not stimulated (Left panel) or stimulated 3 days with anti-mouse CD3 and anti-mouse CD28 (Right panel) and then stained with Brilliant Violet 421™ Rat Anti-Mouse CD4 and SDT Biotin Rat Anti-Mouse CD366 (TIM-3) antibody at 5 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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