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Rat抗原名称
CD86分子别名
T-lymphocyte activation antigen CD86; Activation B7-2 antigen; B70; BU63; CTLA-4 counter-receptor B7.2; FUN-1; CD28LG2细胞定位
Cell membraneAccession
P42081克隆号
GL-1抗体类型
Rat mAb抗体同种型
IgG2a,k反应种属 ?
Ms阳性样本
LPS treated C57BL/6 mouse splenocytes纯化方式
Protein G浓度
0.2 mg/ml标记
APC性状
Liquid缓冲体系
PBS, 1% BSA, 0.09% sodium azide
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 5μl per million cells in 100μl volume Ms
CD86, also known as B7-2, is a co-stimulatory molecule expressed on the surface of antigen-presenting cells (APCs) such as dendritic cells, macrophages, and B cells. It plays a crucial role in the immune response by interacting with CD28 on T cells to provide a necessary co-stimulatory signal for T cell activation, proliferation, and differentiation. Additionally, CD86 can also bind to CTLA-4, an inhibitory receptor on T cells, thereby regulating immune responses and maintaining immune homeostasis. This dual functionality makes CD86 a key target in immunotherapy research, particularly in the development of cancer treatments and autoimmune disease management strategies.
流式分析
Flow cytometric analysis of Mouse CD86/B7-2 expression on LPS treated C57BL/6 mouse splenocytes. C57BL/6 mouse splenocytes (Left) or treated with LPS (500ng/ml, 3d) (Right) were stained with Brilliant Violet 421™ Mouse Anti-Human CD19 antibody and SDT APC Rat Anti-Mouse CD86/B7-2 antibody at 5 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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