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宿主来源
Mouse抗原名称
CD209分子别名
CD209 antigen; C-type lectin domain family 4 member L; Dendritic cell-specific ICAM-3-grabbing non-integrin 1 (DC-SIGN; DC-SIGN1); CLEC4L细胞定位
Cell membraneAccession
Q9NNX6克隆号
S-2881抗体类型
Mouse mAb抗体同种型
IgG2b,k反应种属 ?
Hu阳性样本
Human peripheral blood monocyte-derived dendritic cells纯化方式
Protein A浓度
0.2 mg/ml标记
PerCP-Cy5.5性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 5μl per million cells in 100μl volume Hu
CD209 (also called DC-SIGN) is a C-type lectin transmembrane receptor encoded by the CD209 gene, expressed predominantly on immature dendritic cells and macrophages; its extracellular carbohydrate-recognition domain binds high-mannose glycans on a broad spectrum of pathogens—ranging from HIV-1, HCV, Ebola, Dengue and SARS-CoV to mycobacteria—thereby functioning as a pattern-recognition receptor that triggers pathogen internalization, lysosomal degradation and subsequent MHC-II-restricted antigen presentation to prime T cells, while also mediating dendritic cell rolling on ICAM-2/ICAM-3 for migration and immune-synapse formation, with polymorphisms in its neck region linked to altered susceptibility to HIV-1 and SARS severity.
流式分析
Flow cytometric analysis of Human CD209 expression on Human peripheral blood monocyte-derived dendritic cells. Human peripheral blood monocyte-derived dendritic cells were stained with FITC Mouse Anti-Human CD11c antibody(S0B1731) and either PerCP-Cy5.5 Mouse IgG2a, κ Isotype Control (Left panel) or SDT PerCP-Cy5.5 Mouse Anti-Human CD209 Antibody (Right panel) at 5 μl/test treated with True-Stain Monocyte Blocker™. Total viable cells, as determined by Fixable Viability Dye 452 (S0D0021), were used for analysis. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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