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宿主来源
Rat抗原名称
CD11b分子别名
Integrin alpha-M; CD11 antigen-like family member B; CR-3 alpha chain; Cell surface glycoprotein MAC-1 subunit alpha; Leukocyte adhesion receptor MO1; Itgam细胞定位
Cell membraneAccession
P05555克隆号
M1/70抗体类型
Rat mAb抗体同种型
IgG2b,k反应种属 ?
Hu, Ms阳性样本
C57BL/6 mouse bone marrow纯化方式
Protein G浓度
0.1 mg/ml标记
PE-Cy7性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 1μl per million cells in 100μl volume Hu, Ms
CD11b, also known as integrin alpha M (ITGAM), is a cell surface receptor belonging to the integrin family and primarily expressed on immune cells such as macrophages, monocytes, neutrophils, and NK cells. It forms a heterodimer with CD18 to create the β2 integrin complex, which is crucial for mediating cell adhesion, migration, and immune responses. CD11b plays a significant role in leukocyte recruitment and activation during inflammation and immune responses by interacting with ligands like ICAM-1 and iC3b. Additionally, it is involved in phagocytosis and immune regulation in various diseases, including cancer and autoimmune conditions.
流式分析
Flow cytometric analysis of CD11b expression on C57BL/6 mouse bone marrow. C57BL/6 mouse bone marrow was stained with Pacific Blue™ Rat Anti-Mouse Ly-6G/Ly-6C and either PE-Cy7 Rat IgG2b, κ Isotype Control (Left panel) or SDT PE-Cy7 Rat Anti-Mouse/Human CD11b Antibody (Right panel) at 1 μl/test treated with True-Stain Monocyte Blocker™. Total viable cells, as determined by Fixable Viability Dye 452 (S0D0021), were used for analysis. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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