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宿主来源
Rabbit抗原名称
Ly6G分子别名
Lymphocyte antigen 6G; Ly-6G; Ly-6G.1; Ly6g免疫原
Synthetic Peptide细胞定位
Cell membraneAccession
P35461克隆号
S-2495-6抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Ms阳性样本
C57BL/6 mouse splenocytes纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS pH7.4
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 1:50 Ms
Ly6G (Lymphocyte antigen 6 complex locus G6D) is a 21-25 kDa glycosylphosphatidylinositol (GPI)-anchored protein that belongs to the Ly-6 superfamily and is expressed in a developmentally regulated manner by myeloid cells, including neutrophils, granulocytes, and some monocytes. It serves as a key marker for mature neutrophils and is widely used in research to identify and deplete these cells in vivo. Ly6G is transiently expressed on monocytes during bone marrow development, while its expression on granulocytes and peripheral neutrophils correlates with their differentiation and maturation levels. This antigen plays important roles in immune responses, such as antitumor immunity and neutrophil-mediated inflammation.
流式分析
Flow cytometric analysis of C57BL/6 mouse splenocytes labelling mouse Ly6G antibody at 1/50 (1 μg) dilution/ (Right panel) compared with a Rabbit IgG Isotype Control / (left panel). Goat Anti-Rabbit IgG Alexa Fluor® 647 was used as the secondary antibody. Then cells were stained with CD11b - Brilliant Violet 421™ antibody separately.
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