FITC Rat Anti-Mouse CD16/CD32 Antibody (2.4G2)
Low affinity immunoglobulin gamma Fc region receptor III,IgG Fc receptor III,Fc-gamma RIII (FcRIII),Fcgr3,Low affinity immunoglobulin gamma Fc region receptor II,Fc gamma receptor IIB,Fc-gamma RII,Fc-gamma-RIIB,FcRII,IgG Fc receptor II beta,Lymphocyte antigen 17 (Ly-17),Fcgr2,Fcgr2b,Ly-17
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宿主来源
Rat抗原名称
CD16/CD32分子别名
Low affinity immunoglobulin gamma Fc region receptor III; IgG Fc receptor III; Fc-gamma RIII (FcRIII); Fcgr3; Low affinity immunoglobulin gamma Fc region receptor II; Fc gamma receptor IIB; Fc-gamma RII; Fc-gamma-RIIB; FcRII; IgG Fc receptor II beta; Lymphocyte antigen 17 (Ly-17); Fcgr2; Fcgr2b; Ly-17细胞定位
Cell membraneAccession
P08508, P08101克隆号
2.4G2抗体类型
Rat mAb抗体同种型
IgG2b,k反应种属 ?
Ms阳性样本
C57BL/6 mouse splenocytes纯化方式
Protein G浓度
0.2 mg/ml标记
FITC性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 5μl per million cells in 100μl volume Ms
CD16/CD32 proteins, also known as FcγRIII and FcγRII, are cell surface receptors expressed on immune cells such as natural killer cells, macrophages, and certain T cell subsets; they bind the Fc region of immunoglobulin G to mediate antibody-dependent cellular cytotoxicity, phagocytosis, and immune complex clearance by triggering activating (ITAM-based) or inhibitory (ITIM-based) signaling cascades that modulate innate and adaptive immune responses.
流式分析
Flow cytometric analysis of CD16/CD32 expression on C57BL/6 mouse splenocytes. C57BL/6 mouse splenocytes were stained with either FITC Rat IgG2b, κ Isotype Control (Black line histogram) or SDT FITC Rat Anti-Mouse CD16/CD32 antibody (Red line histogram) at 5 μl/test, cells without incubation with primary antibody and secondary antibody (Blue line histogram) was used as unlabelled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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