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宿主来源
Rat抗原名称
CD14分子别名
Monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein; Cd14细胞定位
Cell membraneAccession
P10810克隆号
Sa14-2抗体类型
Rat mAb抗体同种型
IgG2a,k反应种属 ?
Ms阳性样本
RAW 264.7纯化方式
Protein G浓度
0.2 mg/ml标记
Alexa Fluor® 647性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 1.25μl per million cells in 100μl volume Ms
CD14 is a 375-amino-acon, pattern-recognition glycoprotein that circulates in plasma as a soluble 55 kDa fragment (sCD14) shed by GPI-anchor cleavage or exists as a 55 kDa, glycosylphosphatidylinositol-anchored membrane receptor (mCD14) on myeloid cells; in both forms it acts as the critical co-receptor for Toll-like receptor 4/MD-2, enabling innate immune detection of lipopolysaccharide and other bacterial lipoglycans, thereby triggering NF-κB–driven cytokine storms, and its serum concentration rises acutely in sepsis, making it both a biomarker and a therapeutic target for endotoxin-driven inflammation.
流式分析
Flow cytometric analysis of Mouse CD14 expression on RAW 264.7. Cells from the RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage, Right) or Neuro-2a (Mouse neuroblastoma neuroblast, Left) was stained with Alexa Fluor® 647 Rat IgG2a, κ Isotype Control (Black line histogram) and SDT Alexa Fluor® 647 Rat Anti-Mouse CD14 Antibody (Red line histogram) at 1.25 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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