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宿主来源
Mouse抗原名称
CD303分子别名
C-type lectin domain family 4 member C; Blood dendritic cell antigen 2 (BDCA-2); C-type lectin superfamily member 7; Dendritic lectin; BDCA2; CLECSF11; CLECSF7; DLEC; HECL; CLEC4C细胞定位
Cell membraneAccession
Q8WTT0克隆号
S-R662抗体类型
Mouse mAb抗体同种型
IgG2a,k反应种属 ?
Hu阳性样本
Human PBMC纯化方式
Protein A浓度
0.2 mg/ml标记
Alexa Fluor® 488性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 5μl per million cells in 100μl volume Hu
CD303, also known as Blood Dendritic Cell Antigen 2 (BDCA-2), is a C-type lectin receptor encoded by the CLEC4C gene, selectively expressed on human plasmacytoid dendritic cells (pDCs); it couples via the ITAM‐containing FcεRIγ adaptor to trigger Syk/Ca²⁺ signaling that drives endocytosis, the secretion of large amounts of type I interferon and pro-inflammatory cytokines, and the down-regulation of the pDC interferon response, making it both a key sentinel of viral RNA/DNA and a potential therapeutic target in autoimmunity and allergy.
流式分析
Flow cytometric analysis of Human CD303 expression on human PBMC (human peripheral blood mononuclear cells). Human PBMC were stained with PE/Cy7 Mouse Anti-Human CD123 Antibody and either Alexa Fluor® 488 Mouse IgG2a, κ Isotype Control (Left panel) or SDT Alexa Fluor® 488 Mouse Anti-Human CD303 Antibody (Right panel) at 5μl/test. Total viable cells, as determined by Fixable Viability Dye 452 (S0D0021), were used for analysis. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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