分享产品介绍 评论(0)
分子别名
Monocyte differentiation antigen CD14; My23 antigen; Myeloid cell-specific leucine-rich glycoprotein细胞定位
Secreted, Cell membraneAccession
P08571克隆号
S-3449抗体类型
Mouse mAb抗体同种型
IgG2a,k反应种属 ?
Hu纯化方式
Protein A浓度
0.2 mg/ml标记
PE-Cy7性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 5μl per million cells in 100μl volume Hu
CD14 is a glycosylphosphatidylinositol (GPI)-anchored membrane glycoprotein primarily expressed on monocytes, macrophages, and some granulocytes. It functions as a pattern recognition receptor (PRR) and plays a crucial role in the innate immune response by recognizing bacterial lipopolysaccharides (LPS), pathogen-associated molecular patterns (PAMPs), and damage-associated molecular patterns (DAMPs). CD14 exists in two forms: membrane-bound CD14 (mCD14) and soluble CD14 (sCD14), which can be secreted by activated cells. It acts as a co-receptor for Toll-like receptor 4 (TLR4) and enhances the immune response to LPS by facilitating its delivery to the TLR4-MD2 complex. Additionally, CD14 is involved in TLR4-independent signaling pathways and contributes to tissue homeostasis by promoting the clearance of apoptotic cells.
流式分析
Flow cytometric analysis of Human CD14 expression on human PBMC (human peripheral blood mononuclear cell). Human PBMC were stained with PE-Cy7 Mouse IgG2a, κ Isotype Control (Left panel) or SDT PE-Cy7 Mouse Anti-Human CD14 Antibody (Right panel) at 5 μl/test treated with True-Stain Monocyte Blocker™. Total viable cells, as determined by Fixable Viability Dye 452 (S0D0021), were used for analysis. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
评论(0)