Mouse Anti-Human CD257 Antibody (S-3021)
Tumor necrosis factor ligand superfamily member 13B,B lymphocyte stimulator (BLyS),B-cell-activating factor,BAFF,Dendritic cell-derived TNF-like molecule,TNF- and APOL-related leukocyte expressed ligand 1 (TALL-1),BLYS,TALL1,TNFSF20,ZTNF4,TNFSF13B
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宿主来源
Mouse抗原名称
CD257分子别名
Tumor necrosis factor ligand superfamily member 13B; B lymphocyte stimulator (BLyS); B-cell-activating factor; BAFF; Dendritic cell-derived TNF-like molecule; TNF- and APOL-related leukocyte expressed ligand 1 (TALL-1); BLYS; TALL1; TNFSF20; ZTNF4; TNFSF13B细胞定位
Cell membraneAccession
Q9Y275克隆号
S-3021抗体类型
Mouse mAb抗体同种型
IgG2a,k反应种属 ?
Hu纯化方式
Protein A浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS pH7.4
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 1:400 Hu
CD257, encoded by TNFSF13B and also known as BLyS or BAFF, is a 285-aa type-II transmembrane glycoprotein of the TNF ligand superfamily produced by monocytes, dendritic cells, macrophages and activated T cells that can be cleaved to a soluble homotrimeric cytokine which, via engagement of three receptors—BAFF-R, TACI and BCMA—delivers survival and maturation signals to B cells, orchestrates T-cell co-stimulation, and drives class-switching and antibody production through NF-κB and PI3K/AKT pathways, while excess CD257 triggers B-cell hyperplasia and autoantibody formation, linking its overexpression to systemic lupus erythematosus, Sjögren syndrome, rheumatoid arthritis and multiple myeloma, and making CD257 antagonism (e.g., belimumab) a validated therapeutic strategy.
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流式分析
Flow cytometric analysis of Daudi (Human Burkitt's lymphoma lymphoblast, Left) / U-937 (Human histiocytic lymphoma monocyte, Right) labelling CD257 antibody at 1/400 dilution (0.5 μg) / (Red) compared with a Mouse IgG2a, κ (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Mouse IgG Alexa Fluor® 647 was used as the secondary antibody. Negative control: Daudi
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