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Mouse抗原名称
CD15分子别名
Stage-Specific Embryonic Antigen 1; X-hapten; Lewis X; 3-FAL; SSEA1; SSEA-1细胞定位
Cell membrane克隆号
MC-480抗体类型
Mouse mAb抗体同种型
IgM,k反应种属 ?
Hu, Ms阳性样本
Human peripheral blood cells浓度
0.2 mg/ml标记
PE-Cy7性状
Liquid缓冲体系
PBS, 1% BSA, 0.09% sodium azide
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 1μg per million cells in 100μl volume Hu, Ms
CD15, also known as Lewis X or SSEA-1, is a carbohydrate epitope (Galβ1→4[Fucα1→3]GlcNAc) carried primarily on glycoproteins and glycolipids of myeloid cells, granulocytes, and certain lymphocyte subsets; it serves as a key adhesion molecule that mediates selectin-dependent leukocyte rolling during inflammation, acts as a marker of mature neutrophils and Hodgkin/Reed-Sternberg cells, and its differential expression is exploited in immunohistochemistry to distinguish myeloid leukemias from lymphoid malignancies and to identify stem-cell populations in normal and neoplastic tissues.
流式分析
Flow cytometric analysis of CD15 expression on human peripheral blood cells. Human peripheral blood cells were stained with PE-Cy7 Mouse IgM,k Isotype Control (left panel) or SDT PE-Cy7 Mouse Anti-Human CD15 Antibody (right panel) at 5 μl/test with True-Stain Monocyte Blocker™. Total viable cells, as determined by Fixable Viability Dye 452 (S0B88801), were used for analysis. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
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